Active Recombinant Human EGFL6 Protein, His-tagged

• Cat.No.: EGFL6-22H
• Product Overview: Recombinant Human EGF-L6 (Asn22-Arg363) Protein with a His tag at C-terminal was expressed in HEK293 Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-363 a.a.
• Description: This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. The gene product contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. This gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148. The ED50 for this effect is 1-5 μg/mL.
• Molecular Mass: 40 kDa
• N-terminal Sequence Analysis: Asn22
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: EGFL6 EGF like domain multiple 6 [ Homo sapiens (human) ]
• Official Symbol: EGFL6 EGF like domain multiple 6 [ Homo sapiens (human) ]
• Synonyms: EGFL6; EGF like domain multiple 6; W80; MAEG; epidermal growth factor-like protein 6; EGF repeat-containing protein 6; EGF-like protein 6; MAM and EGF domain containing; MAM and EGF domains-containing gene protein
• Gene ID: 25975
• mRNA Refseq: NM_015507
• Protein Refseq: NP_056322
• MIM: 300239
• UniProt ID: Q8IUX8