Active Recombinant Human EEF2K, GST-tagged

• Cat.No.: EEF2K-1398H
• Product Overview: Recombinant full-length human EEF2K was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: EEF2K is a highly conserved calmodulin-dependent protein kinase that links activation of cell surface receptors to cell division. EEF2K is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2), an abundant cytoplasmic protein that catalyzes the movement of the ribosome along mRNA during translation in eukaryotic cells, and inhibits the EEF2 function. EEF2K is highly expressed in heart and skeletal muscle, suggesting that EEF2 phosphorylation may be particularly important in muscle. EEF2K is highly expressed in patients with systemic lupus erythematosus as well as in many cancers.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 50 nmol/min/mg
• Molecular Mass: ~125 kDa
• Protein length: Full length
• Purity: >80%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: EEF2K eukaryotic elongation factor-2 kinase [ Homo sapiens ]
• Official Symbol: EEF2K
• Synonyms: EEF2K; eukaryotic elongation factor-2 kinase; eukaryotic elongation factor 2 kinase; eEF 2K; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; eEF-2K; HSU93850; MGC45041
• Gene ID: 29904
• mRNA Refseq: NM_013302
• Protein Refseq: NP_037434
• MIM: 606968
• UniProt ID: O00418
• Chromosome Location: 16p12
• Pathway: IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem; PI3K Cascade, organism-specific biosystem; PKB-mediated events, organism-specific biosystem; S6K1 signalling, organism-specific biosystem; S6K1-mediated signalling, organism-specific biosystem
• Function: ATP binding; binding; calcium ion binding; calmodulin binding; elongation factor-2 kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; translation factor activity, nucleic acid binding