Active Recombinant Human DYRK2 Protein, GST-tagged

• Cat.No.: DYRK2-2986H
• Product Overview: Human DYRK2 (NP_003574.1, 1 a.a. - 528 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008]
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: DYRKtide-F.
  ATP: 100 μM.
• Molecular Mass: 87 kDa
• Purity: 98 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: DYRK2 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 [ Homo sapiens ]
• Official Symbol: DYRK2
• Synonyms: DYRK2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; dual specificity tyrosine-phosphorylation-regulated kinase 2; FLJ21217; FLJ21365
• Gene ID: 8445
• mRNA Refseq: NM_003583
• Protein Refseq: NP_003574
• MIM: 603496
• UniProt ID: Q92630