Active Recombinant Human DSG2, Fc Chimera

• Cat.No.: DSG2-512H
• Product Overview: Recombinant Human DSG2 (Accession # CAA81226) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala49
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 3 x 10^4 cells are added to recombinant human Desmoglein-2 coated plates (10 µg/mL, 100 µL/well), approximately 50%-80% will adhere after 1 hour at 37°C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human DSG2, Fc Chimera has a calculated MW of 89 kDa (monomer). In SDS-PAGE migrates as 115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: DSG2 desmoglein 2 [ Homo sapiens ]
• Official Symbol: DSG2
• Synonyms: DSG2; desmoglein 2; desmoglein-2; CDHF5; HDGC; cadherin family member 5; ARVC10; ARVD10; CMD1BB; MGC117034; MGC117036; MGC117037
• Gene ID: 1829
• mRNA Refseq: NM_001943
• Protein Refseq: NP_001934
• MIM: 125671
• UniProt ID: Q14126
• Chromosome Location: 18q12.1