Active Recombinant Human DOT1L protein, GST-tagged

• Cat.No.: DOT1L-180H
• Product Overview: Recombinant Human DOT1L(1-435 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-435 aa
• Description: DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones. DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 14 nmol/min/mg
• Molecular Mass: 78 kDa
• Purity: >90%
• Applications: Methyltransferase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: DOT1L DOT1-like, histone H3 methyltransferase (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DOT1L
• Synonyms: DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K7
• Gene ID: 84444
• mRNA Refseq: NM_032482
• Protein Refseq: NP_115871
• MIM: 607375
• UniProt ID: Q8TEK3
• Chromosome Location: 19p13.3
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transcription factor binding; transferase activity