Active Recombinant Human DLL1 protein, His-tagged

• Cat.No.: DLL1-278H
• Product Overview: Recombinant Human DLL1(NP_005609)(Gln 18 - Gly 540), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln 18 - Gly 540
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human NOTCH1, Fc Tag on Protein A Biosensor, can bind Human DLL1, His Tag with an affinity constant of 290 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: The protein has a calculated MW of 58.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: DLL1 delta-like 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL1
• Synonyms: DLL1; delta-like 1 (Drosophila); delta (Drosophila) like 1; delta-like protein 1; H-Delta-1; drosophila Delta homolog 1; DL1; Delta; DELTA1
• Gene ID: 28514
• mRNA Refseq: NM_005618
• Protein Refseq: NP_005609
• MIM: 606582
• UniProt ID: O00548