Recombinant Rat Dll1 protein(Met1-Ser534), hFc-tagged

• Cat.No.: Dll1-548R
• Product Overview: Recombinant Rat DLL1 (EDL99825.1) (Met1-Ser534) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ser534
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-18 μg/mL in the presence of 500 ng/mL recombinant human BMP2.
• Molecular Mass: The recombinant rat DLL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 752 amino acids and has a predicted molecular mass of 82.4 kDa. The apparent molecular mass of the protein is approximately 105.1, 57, 38.7 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Dll1 delta-like 1 (Drosophila) [ Rattus norvegicus ]
• Official Symbol: Dll1
• Synonyms: DLL1; delta-like 1 (Drosophila); delta-like protein 1; delta1; drosophila Delta homolog 1
• Gene ID: 84010
• mRNA Refseq: NM_032063
• Protein Refseq: NP_114452