Recombinant Human DKK1 protein, hFc-Avi-tagged

• Cat.No.: DKK1-1700H
• Product Overview: Recombinant Human DKK1 protein(O94907)(Met178-His266), fused with C-terminal hFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-hFc-Avi
• Protein Length: Met178-His266
• Tag: C-hFc-Avi
• Form: Supplied as 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0).
• Bio-activity: Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907