Active Recombinant Human CYP2A13 Protein

• Cat.No.: CYP2A13-34H
• Product Overview: Recombinant human CYP2A13 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. Although its endogenous substrate has not been determined, it is known to metabolize 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a major nitrosamine specific to tobacco. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
• Bio-activity: Cytochrome c Reductase Activity: 39 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Coumarin 7-hydroxylase
  Linear with time up to: 15 min(Determined at 0.2 µM substrate, using a P450 concentration of 10 pmol/ml)
  Linear with P450 up to: 30 pmol/ml(Determined at 0.2 µM substrate, using an incubation time of 5 min)
  Vmax: 0.25 pmol/min/pmol CYP
  Km: 0.44 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2A13 cytochrome P450 family 2 subfamily A member 13 [ Homo sapiens (human) ]
• Official Symbol: CYP2A13
• Synonyms: CYP2A13; cytochrome P450 family 2 subfamily A member 13; CPAD; CYP2A; CYPIIA13; cytochrome P450 2A13; cytochrome P450, family 2, subfamily A, polypeptide 13; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 13; EC 1.14.14.1
• Gene ID: 1553
• mRNA Refseq: NM_000766
• Protein Refseq: NP_000757
• MIM: 608055
• UniProt ID: Q16696