Active Recombinant Human CTSZ Protein, His-tagged

• Cat.No.: CTSZ-191H
• Product Overview: Recombinant human CTSZ (Q9UBR2) (Met 1-Val 303) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: 1-303 a.a.
• Predicted N Terminal: Gly 24
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH.The specific activity is >800 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human CTSZ (pro form) consists of 291 amino acids and has a predicted molecular mass of 33 kDa. The apparent molecular mass of rhCTSZ is approximately 40 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CTSZ cathepsin Z [ Homo sapiens ]
• Official Symbol: CTSZ
• Synonyms: CTSZ; cathepsin Z; carboxypeptidase LB; cathepsin B2; cathepsin IV; cathepsin X; cathepsin Y; cathepsin Z1; CTSX; cysteine type carboxypeptidase; lysosomal carboxypeptidase B; cathepsin P; preprocathepsin P; cysteine-type carboxypeptidase; FLJ17088
• Gene ID: 1522
• mRNA Refseq: NM_001336
• Protein Refseq: NP_001327
• MIM: 603169
• UniProt ID: Q9UBR2
• Chromosome Location: 20q13.32
• Pathway: Clathrin derived vesicle budding, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Lysosome Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem