Active Recombinant Human CTSB protein, His-tagged

• Cat.No.: CTSB-169H
• Product Overview: Recombinant Human CTSB protein(NP_001899.1)(Arg18-Ile339), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Arg18-Ile339
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is >2,500 pmoles/min/μg.
• Molecular Mass: The recombinant human CTSB existing as the proform consists of 332 amino acids and has a predicted molecular mass of 37.2 kDa. rhCTSB migrates with the molecular weight of 36 and 43 kDa as the proform and mature form rspectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTSB cathepsin B [ Homo sapiens ]
• Official Symbol: CTSB
• Synonyms: CTSB; cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase; APPS; CPSB
• Gene ID: 1508
• mRNA Refseq: NM_001908
• Protein Refseq: NP_001899
• MIM: 116810
• UniProt ID: P07858