Active Recombinant Human CSTA protein, His-tagged

• Cat.No.: CSTA-203H
• Product Overview: Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
• Bio-activity: The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
• Molecular Mass: 13.1 kDa (118aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVFKSLPGQNEDL VLTGYQVDKN KDDELTGF
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by BCA assay)

Gene Information

• Gene Name: CSTA cystatin A (stefin A) [ Homo sapiens ]
• Official Symbol: CSTA
• Synonyms: CSTA; cystatin A (stefin A); STF1; STFA; CSTA; Cystatin-A; Cystatin-AS; cystatin A; cystatin AS; stefin A
• Gene ID: 1475
• mRNA Refseq: NM_005213
• Protein Refseq: NP_005204
• MIM: 184600
• UniProt ID: P01040
• Chromosome Location: 3q21
• Function: cysteine-type endopeptidase inhibitor activity; protease binding; protein binding, bridging; structural molecule activity