Active Recombinant Human CSK, GST-tagged

• Cat.No.: CSK-1394H
• Product Overview: Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• ProteinLength: Full length
• Description: CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src through tyrosine phosphorylation of the c-src carboxy terminus. A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells. This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 425 nmol/min/mg
• Molecular Mass: ~78 kDa
• Purity: >85%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: CSK c-src tyrosine kinase [ Homo sapiens ]
• Official Symbol: CSK
• Synonyms: CSK; c-src tyrosine kinase; tyrosine-protein kinase CSK; C-Src kinase; protein-tyrosine kinase CYL; MGC117393
• Gene ID: 1445
• mRNA Refseq: NM_001127190
• Protein Refseq: NP_001120662
• MIM: 124095
• UniProt ID: P41240
• Chromosome Location: 15q24.1
• Pathway: Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Disease, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem
• Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; proline-rich region binding; protein C-terminus binding; protein binding; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine phosphatase activity