Active Recombinant Human CLDN4 Full Length Transmembrane protein, His&Strep-tagged(Nanodisc)

• Cat.No.: CLDN4-257H
• Product Overview: Active Recombinant Human CLDN4 Full Length Transmembrane protein(O14493) (Met1-Val209) was expressed in HEK293, with a polyhistidine tag at the N-terminus and a Strep tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Strep
• Protein Length: Met1-Val209
• Form: Supplied as sterile 10mM Hepes, 50mM NaCl, 1% Glycerol, pH 7.5.
• Bio-activity: Immobilized Human Claudin-4-Nanodisc Protein, His, Strep tag at 5 μg/mL (100 μL/well) can bind Anti-Claudin-4 Antibody,human IgG1, the EC50 is 10-31 ng/mL.
• Molecular Mass: The recombinant Human CLDN4-Nanodisc consists of 228 amino acids and predicts a molecular mass of 24.62 kDa. It migrates as an approximately 53.2 kDa 21.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CLDN4 claudin 4 [ Homo sapiens ]
• Official Symbol: CLDN4
• Synonyms: CLDN4; claudin 4; CPETR, CPETR1; claudin-4; Clostridium perfringens enterotoxin receptor 1; CPE R; hCPE R; WBSCR8; Williams Beuren syndrome chromosomal region 8 protein
• Gene ID: 1364
• mRNA Refseq: NM_001305
• Protein Refseq: NP_001296
• MIM: 602909
• UniProt ID: O14493