Active Recombinant Human CDSN, His-tagged

• Cat.No.: CDSN-407H
• Product Overview: Recombinant Human CDSN (Accession # AAN70994) Lys33-Pro528, fused with a C-terminal 6-His tag (Asp526Asn), was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 33-528 a.a.
• Predicted N Terminal: Lys33
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 3 x 10^4 cells/well are added to rhCDSN coated plates (10 µg/mL, 100 µL/well), approximately 50 - 80% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human CDSN, His-tagged has a calculated MW of 49.1 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CDSN corneodesmosin [ Homo sapiens ]
• Official Symbol: CDSN
• Synonyms: CDSN; corneodesmosin; D6S586E; differentiated keratinocyte S protein; S; PSS; HTSS; HTSS1
• Gene ID: 1041
• mRNA Refseq: NM_001264
• Protein Refseq: NP_001255
• MIM: 602593
• UniProt ID: Q15517
• Chromosome Location: 6p21.3