Active Recombinant Human CDK9/CCNK protein, GST-tagged

• Cat.No.: CDK9-26809TH
• Product Overview: Recombinant full-length human CDK9 and CCNK were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: CDK9/CCNK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle. CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 18nmol/min/mg
• Molecular Mass: CDK9~68 kDa; CCNK~67 kDa
• Purity: The purity of CDK9/CCNK was determined to be >85% by densitometry.
• Applications: Kinase Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: cyclin dependent kinase 9 [ Homo sapiens (human) ]
• Official Symbol: CDK9
• Synonyms: TAK; C-2k; CTK1; CDC2L4; PITALRE; CPR4
• Gene ID: 1025
• mRNA Refseq: NM_001261
• Protein Refseq: NP_001252
• MIM: 603251
• UniProt ID: P50750