Active Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein (Ala2-Phe346 & Tyr2-Leu323 & Asp2-Ser309), His-tagged

• Cat.No.: CCM-1029H
• Product Overview: Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein (P50613)(Ala2-Phe346) & (P51946)(Tyr2-Leu323) & (P51948)(Asp2-Ser309) was produced by Baculovirus-Insect Cells expression system. This protein was fused with a polyhistidine tag at the N-terminus, constructed the plasmid. The three plasmids were co-expressed and the heterotrimer was purified.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: Ala2-Phe346 & Tyr2-Leu323 & Asp2-Ser309
• Predicted N Terminal: His & His & His
• Form: Supplied as sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: The specific activity was determined to be 18 nmol/min/mg using MBP as substrate.
• Molecular Mass: The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25,38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
  Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Gene Information

• Gene Name: CDK7 cyclin-dependent kinase 7 [ Homo sapiens ] & CCNH cyclin H [ Homo sapiens ] & MNAT1 menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: CDK7 & CCNH & MNAT1
• Synonyms: CDK7; CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15; HCAK; p39MO15 & CCNH; cyclin H; cyclin-H; p34; p37; CAK & MAT1; TFB3; CAP35; RNF66
• Gene ID: 1022 & 902 & 4331
• mRNA Refseq: NM_001799 & NM_001199189 & NM_002431.3
• Protein Refseq: NP_001790 & NP_001186118 & NP_002422.1
• MIM: 601955 & 601953 & 602659
• UniProt ID: P50613 & P51946 & P51948