Active Native Bovine Myosin Protein

• Cat.No.: MYH-10B
• Product Overview: Cardiac myosin protein has been purified from bovine heart muscle. The full length myosin protein has been purified with its essential light chains (ELC) and regulatory light chains (RLC)

Specification

• Species: Bovine
• Source: Bovine heart muscle
• Tag: Non
• Form: Lyophilized from 100 mM Imidizole pH 7.5, 2.4 M KCl, 0.2 mM DTT, 5% sucrose and 1% dextran.
• Bio-activity: The biological activity of cardiac myosin is determined from its rate of F-actin activated ATP hydrolysis. A standard biological assay for monitoring ATP hydrolysis by myosin consists of an in vitro F-actin ATPase assay. Stringent quality control ensures that in the presence of F-actin, rabbit myosin will have a minimum hydrolysis rate 10 fold greater than in the absence of F-actin, which is comparable to published results. Rabbit myosin is also available for comparison studies.
• Molecular Mass: The myosin heavy chain is located at approximately 200 kDa, whereas the RLC is approximately 20 kDa and the ELC isoform is approximately 25 kDa.
• Purity: 95%
• Stability: The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for 1 year.
• Reconstitution: Reconstituted in nanopure water.
• Full Length: Full L.

Gene Information

• Synonyms: Myosin; Cardiac myosin; Myosin light chains