Active Recombinant Human CDK6/CycD3 Protein, GST/His-tagged

• Cat.No.: CDK6/CycD3-3549H
• Product Overview: Recombinant Human CDK6/CycD3 Protein is produced by Sf9 insect cells expression system. Human CDK6, amino acids M1-A326, N-terminal GST fusion protein with a Thrombin cleavage site and human CycD3, amino acids M1-L292, N-terminally fused to HIS6-Thrombin cleavage site.

Specification

• Species: Human
• Source: Insect cells
• Tag: His&GST
• ProteinLength: CDK6 (M1-A326) and CycD3 (M1-L292)
• Form: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol.
• Bio-activity: Specific kinase activity (Pi transfer): 65 pmol/µg × min. ATP-KM: 43 µM.
• Molecular Mass: GST-CDK6 (63,279 Da) and CycD3 (37,260 Da)
• AA Sequence: CDK6: MEKD GLCRADQQYE CVAEIGEGAY GKVFKARDLK NGGRFVALKR VRVQTGEEGM PLSTIREVAV LRHLETFEHP NVVRLFDVCT VSRTDRETKL TLVFEHVDQD LTTYLDKVPE PGVPTETIKD MMFQLLRGLD FLHSHRVVHR DLKPQNILVT SSGQIKLADF GLARIYSFQM ALTSVVVTLW YRAPEVLLQS SYATPVDLWS VGCIFAEMFR RKPLFRGSSY VDQLGKILDV IGLPGEEDWP RDVALPRQAF HSKSAQPIEK FVTDIDELGK DLLLKCLTFN PAKRISAYSA LSHPYFQDLE RCKENLDSHL PPSQNTSELN TA MEKDGLCRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTG EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDVIGLPGE EDWPRDVALP RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYSALSHPYF QDLERCKENL DSHLPPSQNT SELNTA
  CycD3: MELLC CEGTRHAPRA GPDPRLLGDQ RVLQSLLRLE ERYVPRASYF QCVQREIKPH MRKMLAYWML EVCEEQRCEE EVFPLAMNYL DRYLSCVPTR KAQLQLLGAV CMLLASKLRE TTPLTIEKLC IYTDHAVSPR QLRDWEVLVL GKLKWDLAAV IAHDFLAFIL HRLSLPRDRQ ALVKKHAQTF LALCATDYTF AMYPPSMIAT GSIGAAVQGL GACSMSGDEL TELLAGITGT EVDCLRACQE QIEAALRESL REAAQTSSSP APKAPRGSSS QGPSQTSTPT DVTAIHL MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR EIKPHMRKML AYWMLEVCEE QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ LLGAVCMLLA SKLRETTPLT IEKLCIYTDH AVSPRQLRDW EVLVLGKLKW DLAAVIAHDF LAFILHRLSL PRDRQALVKKHAQTFLALCA TDYTFAMYPP SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA LRESLREASQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL
• Storage: Store at -80 centigrade.
  For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles.
• Concentration: 0.738 µg/µl

Gene Information

• Gene Name: CDK6 cyclin-dependent kinase 6 [ Homo sapiens ]
• Official Symbol: CDK6
• Synonyms: CDK6; cyclin-dependent kinase 6; PLSTIRE; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; MGC59692
• Gene ID: 1021
• mRNA Refseq: NM_001145306
• Protein Refseq: NP_001138778
• MIM: 603368
• UniProt ID: Q00534