Active Recombinant Human CD96 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD96-3888H
• Product Overview: Biotinylated Human CD96, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Val 22 - Met 503 (Accession # P40200-2). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 22-503 a.a.
• Predicted N Terminal: Val 22
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Biotinylated Human CD96, His Tag, Avi Tag at 5 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 39-625 ug/mL.
• Molecular Mass: The protein has a calculated MW of 57.3 kDa. The protein migrates as 120-130 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD96 CD96 molecule [ Homo sapiens ]
• Official Symbol: CD96
• Synonyms: CD96; CD96 molecule; CD96 antigen; T-cell surface protein tactile; TACTILE; cell surface antigen CD96; T cell activation, increased late expression; t cell-activated increased late expression protein; MGC22596; DKFZp667E2122
• Gene ID: 10225
• mRNA Refseq: NM_005816
• Protein Refseq: NP_005807
• MIM: 606037
• UniProt ID: P40200