Active Recombinant Human CD40LG Protein (116-261), N-FLAG tagged, Biotinylated

• Cat.No.: CD40LG-11H
• Product Overview: Recombinant human CD40L (aa 116-261) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG-tag and Biotin labeled was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Flag
• Protein Length: 116-261
• Description: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
• Form: Lyophilized
• Bio-activity: Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: < 1 ng/mL).
• Molecular Mass: ~35-40 kDa (SDS-PAGE)
• Endotoxin: < 0.01 EU/μg purified protein (LAL test, Lonza).
• Purity: ≥95 % (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -20 centigrade.
  Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage: +4 centigrade
  Long Term Storage: -20 centigrade
• Concentration: 0.1 mg/mL after reconstitution.
• Storage Buffer: Contains PBS
• Reconstitution: Reconstitute with 100 μL sterile water.
• Handling: After reconstitution, prepare aliquots and store at -20 centigrade.
  Avoid freeze/thaw cycles.
  Centrifuge lyophilized vial before opening and reconstitution.
  PBS containing at least 0.1 % BSA should be used for further dilutions.
• Shipping: BLUE ICE

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens (human) ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD40 ligand; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965