Active Recombinant Human CD40LG Protein, Fc/Avi-tagged, Biotinylated, Alexa Fluor 488 conjugated

• Cat.No.: CD40LG-280HAF488
• Product Overview: Biotinylated, Alexa Fluor 488 conjugated Human CD40 Ligand, Fc Tag is expressed from HEK293 cells. It contains AA Met 113 - Leu 261 (Accession # P29965-1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 113-261 a.a.
• Form: Lyophilized
• Bio-activity: Immobilized Human CD40, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand, Fc Tag with a linear range of 1-31 μg/mL.
• Molecular Mass: The protein has a calculated MW of 45.0 kDa. The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• N-terminal Sequence Analysis: Gly
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965