Active Recombinant Human CCNA1 protein, His-tagged

• Cat.No.: CCNA1-3335H
• Product Overview: Recombinant Human CCNA1 protein(NP_003905.1)(Met1-Gln465), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Gln465
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.
• Molecular Mass: The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CCNA1 cyclin A1 [ Homo sapiens ]
• Official Symbol: CCNA1
• Synonyms: CCNA1; cyclin A1; cyclin-A1
• Gene ID: 8900
• mRNA Refseq: NM_001111045
• Protein Refseq: NP_001104515
• MIM: 604036
• UniProt ID: P78396