Active Recombinant Human BTC protein(Met1-Tyr111), hFc-tagged

• Cat.No.: BTC-395H
• Product Overview: Recombinant Human BTC (P35070) (Met1-Tyr111) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Tyr111
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell prolifeRation assay using Balb/3T3 mouse embryonic fibroblast cells.The ED50 for this effect is typically 5-40 ng/mL.
• Molecular Mass: The recombinant human BTC/Fc is a disulfide-linked homodimer. The reduced monomer comprises 321 amino acids and has a predicted molecular mass of 36 kDa. The apparent molecular mass of the protein is approximately 43-46 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTC betacellulin [ Homo sapiens ]
• Official Symbol: BTC
• Synonyms: BTC; betacellulin; probetacellulin
• Gene ID: 685
• mRNA Refseq: NM_001729
• Protein Refseq: NP_001720
• MIM: 600345
• UniProt ID: P35070