Active Recombinant Human BMP4 Protein, Animal Free

• Cat.No.: BMP4-04H
• Product Overview: Recombinant Human BMP4 Protein without tag was expressed in Human cells.
  Animal Free and Xeno Free

Specification

• Description: Bone morphogenetic protein 4 (BMP-4) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-4 is widely expressed from early embryogenesis through dulthood. It plays an important role in cartilage and bone formation, mesoderm induction, tooth development, limb formation and fracture repair. Mature human BMP-4 is a 116 amino acids glycoprotein and normally found as a homodimer. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.
• Source: Human cells
• Species: Human
• Bio-activity: The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).
• Molecular Mass: 34-40 kDa
• Endotoxin: <0.5 EU/ μg
• Purity: High Purity, >95% by SDS-PAGE
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein
• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens (human) ]
• Official Symbol: BMP4
• Synonyms: BMP4; bone morphogenetic protein 4; BMP2B; BMP-4; BMP-2B; bone morphogenetic protein 2B; ZYME; OFC11; BMP2B1; MCOPS6
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644