Active Recombinant Human BCL2L11 Protein, His-tagged

• Cat.No.: BCL2L11-572H
• Product Overview: Recombinant Human BCL2L11 (Accession # NP_006529) Ala2-Arg120, fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in HEPES-KOH, DTT, KCl and Glycerol.
• Bio-activity: Measured by its ability to neutralize anti-apoptotic Bcl-2 family member inhibition of Recombinant Human BID Caspase-8-cleaved-mediated Cytochrome c release from isolated mitochondria. Release of Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine ELISA.The typical EC50 value in this assay is 0.1-2 µM. Optimal dose should be determined for each application.
• Molecular Mass: Recombinant Human BCL2L11, His-tagged has a calculated MW of 14.3 kDa. In SDS-PAGE migrates as 16 kDa doublet, under reducing and non-reducing conditions.
• Protein length: Ala2-Arg120
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: BCL2L11 BCL2-like 11 (apoptosis facilitator) [ Homo sapiens ]
• Official Symbol: BCL2L11
• Synonyms: BCL2L11; BCL2-like 11 (apoptosis facilitator); bcl-2-like protein 11; BIM; BimEL; BimL; BOD; bcl2-L-11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death; BAM; BIM-beta6; BIM-beta7; BIM-alpha6
• Gene ID: 10018
• mRNA Refseq: NM_001204106
• Protein Refseq: NP_001191035
• MIM: 603827
• UniProt ID: O43521
• Chromosome Location: 2q13