Active Recombinant Human ATG3 Protein, His-tagged

• Cat.No.: ATG3-744H
• Product Overview: Recombinant Human ATG3 (Accession # NP_071933), fused with a C-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human His6-Apg3p/ATG3/Apg3L is a member of the Apg-conjugating (E2) enzyme family that receives Apg from a Apg-activating (E1) enzyme and subsequently interacts with a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg3p/ATG3/Apg3L concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human ATG3, His-tagged has a calculated MW of 37 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ATG3 ATG3 autophagy related 3 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG3
• Synonyms: ATG3; ATG3 autophagy related 3 homolog (S. cerevisiae); APG3 autophagy 3 like (S. cerevisiae) , APG3L; ubiquitin-like-conjugating enzyme ATG3; DKFZp564M1178; FLJ22125; MGC15201; PC3 96; hApg3; 2610016C12Rik; autophagy-related protein 3; APG3; APG3L; PC3-96; APG3-LIKE
• Gene ID: 64422
• mRNA Refseq: NM_022488
• Protein Refseq: NP_071933
• MIM: 609606
• UniProt ID: Q9NT62
• Chromosome Location: 3q13.2