Active Recombinant Human APOE4 protein, His-tagged

• Cat.No.: APOE4-2866H
• Product Overview: Recombinant Human APOE4 protein(Lys 19-His 317), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Human LDL R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human APOE2, His Tag with an affinity constant of 16.2 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 36.1 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Lys 19-His 317
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.