Active Recombinant Human ANXA1 protein, His-tagged

• Cat.No.: ANXA1-33H
• Product Overview: Recombinant Human ANXA1(Met1-Asn346) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Ala2
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED50 for this effect is typically 1.0-4.0 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 39.4 kDa;SDS-PAGE: 38 kDa, reducing conditions.
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in PBS.
• Protein length: 1-346 a.a.

Gene Information

• Gene Name: ANXA1 annexin A1 [ Homo sapiens ]
• Official Symbol: ANXA1
• Synonyms: ANXA1; annexin A1; ANX1, LPC1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein; ANX1; LPC1
• Gene ID: 301
• mRNA Refseq: NM_000700
• Protein Refseq: NP_000691
• MIM: 151690
• UniProt ID: P04083
• Chromosome Location: 9q21.13
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Formyl peptide receptors bind formyl peptides and many other ligands, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; phospholipase inhibitor activity; phospholipase inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; protein homodimerization activity; receptor binding; single-stranded DNA binding; single-stranded RNA binding; structural molecule activity