Active Recombinant Human ADAMTS5, His-tagged

• Cat.No.: ADAMTS5-534H
• Product Overview: Recombinant Human ADAMTS5 was expressed in abaculovirussystem and purified from insect cells. The enzyme contains the catalytic domain, the disintegrin domain and the thrombospondin type-1 motif of full-length ADAMTS-5 followed by a C-terminal His tag, 50 kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: Aggrecanases belong to a family of ADAMTS proteases (ADAMTS = A disintegrin and metalloprotease with thrombospondin motifs). ADAMTS enzymes are comprised of an N-terminal prodomain, a catalytic domain, a disintegrin domain, one or more thrombospondin (TS) motifs, a cysteine-rich domain and a spacer domain of variable length. The prodomains are cleaved off intracellularly and the enzymes are processed further from their C-terminal ends after secretion. Aggrecanases are responsible for aggrecan degradation in osteoarthritic joints. ADAMTS-5 was found to be the principal aggrecanase in mouse cartilage.
• Activity: Activity (mU/mg) is specified on the vial.
• Purity: ≥ 50%.
• Unit Definition: One milliunit of protease hydrolyzes 1 nanomole of aggrecan interglobular domain substrate per minute at pH 7.5 at 37°C.
• Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 50 mM Imidazol, 0.05% Brij-35.
• Application: Used as a positive control in FRET-based and aggrecan enzyme activity assays, Western blot, IP, and ELISA.
• Storage: Store at -80°C. Avoid multiple thaw-freeze cycles.

Gene Information

• Gene Name: ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [ Homo sapiens ]
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADMP-2; ADAMTS11; FLJ36738; ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS5; ADAM-TS 5; ADAMTS-11;ADAM-TS 11; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); EC 3.4.24.-
• Gene ID: 11096
• mRNA Refseq: NM_007038
• Protein Refseq: NP_008969
• MIM: 605007
• Chromosome Location: 21q21.3
• Function: integrin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding