Recombinant Human ADAMTS5 protein, His & T7-tagged

• Cat.No.: ADAMTS5-1755H
• Product Overview: Recombinant Human ADAMTS5 aa. (Glu485~Pro622 (Accession # Q9UNA0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.0kDa
• Protein length: Glu485~Pro622
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.3

Gene Information

• Gene Name: ADAMTS5 ADAM metallopeptidase with thrombospondin type 1 motif 5 [ Homo sapiens (human) ]
• Official Symbol: ADAMTS5
• Synonyms: ADAMTS5; ADAM metallopeptidase with thrombospondin type 1 motif 5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; ADAM-TS 5; ADAMTS-11; ADAM-TS 11; A disintegrin and metalloproteinase with thrombospondin motifs 5; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); aggrecanase-2
• Gene ID: 11096
• mRNA Refseq: NM_007038.4
• Protein Refseq: NP_008969.2
• UniProt ID: Q9UNA0