Active Recombinant Human ADAM Metallopeptidase With Thrombospondin type 1 Motif, 15, His-tagged

• Cat.No.: ADAMTS15-528H
• Product Overview: Recombinant Human ADAMTS15protein was expressed in Chinese Hamster Ovary cell line with a C-terminal 10-Histag. Gly18-Cys682 (pro) & Phe213-Cys682 (mature).

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 18-682;213-682 a.a.
• Description: ADAMTS (adisintegrin and metalloproteinase with thrombospondin motifs) is a group ofnineteen secreted zinc binding metalloproteases that is involved indevelopment, inflammation, angiogenesis and several pathological conditions.They are synthesized as zymogens which have a prodomain that is removed byfurinlike protein convertases. The mature secreted enzymes consist ofcatalytic, disintegrinlike, type I thrombospondin (TSP) motif, cysteine rich,and spacer domains followed by zero to fourteen additional TSP motifs.
• Form: Supplied as a 0.2 μmfiltered solution in MES and NaCl.
• N-terminalSequence: Gly18 & Phe213
• Molecular Weight: 74 kDa (pro), 53 kDa(mature)
• Activity: Measured by itsability to cleave rhAggrecan. Cleavage of 1 μg of rhAggrecan is > 90%, asmeasured under the described conditions.
• Endotoxin Level: < 1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 85 kDa and 65 kDa,reducing conditions.
• Purity: > 80%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mL in sterilePBS.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditionsafter opening.

Gene Information

• Gene Name: ADAMTS15 ADAM metallopeptidasewith thrombospondin type 1 motif, 15 [ Homo sapiens ]
• Official Symbol: ADAMTS15
• Synonyms: ADAMTS15; ADAMmetallopeptidase with thrombospondin type 1 motif, 15; Adisintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15;ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondindomains; a disintegrin-like and metalloprotease (reprolysin type) withthrombospondin type 1 motif, 15, preproprotein
• Gene ID: 170689
• mRNA Refseq: NM_139055
• Protein Refseq: NP_620686
• MIM: 607509
• UniProt ID: Q8TE58
• Chromosome Location: 11q25
• Function: metal ion binding;metalloendopeptidase activity; peptidase activity; zinc ion binding