Active Recombinant Human Activin A, His-tagged

• Cat.No.: Activin A-15H
• Product Overview: recombinant Human Activin A was produced by transient expression in Nicotiana plant.

Specification

• Description: Activin A is a non-glycosylated cytokine that belongs to the TGF? superfamily. It is involved in a wide variety of biological processes including hematopoiesis, immune response, neural cell differentiation, wound repair and morphogenesis. The Activins form homodimers (e.g. AA; BB) and heterdimers (e.g. AB) and can activate the Smad pathway by signaling through heterodimeric typeI/typeII serine/threonine kinase transmembrane receptors. Activins are also important for production and regulation of several hormones such as FSH, Prolactin, ACTH and oxytocin. Activin A is synthesized by a wide spectrum of cells including bone marrow cells, skin cells, vascular smooth muscle cells and cells of the reproductive system.
• Source: Nicotiana plant
• Species: Human
• Tag: His
• Form: Lyophilized from Tris HCl at pH 7.4.
• Bio-activity: Bioactivity is assayed by measuring the ability of recombinant human Activin A to inhibit proliferation of mouse plasma-cytoma cell line (MPC-11). The ED50 value for this effect is typically below 5 ng/ml.
• Molecular Mass: Dimer of 27,4 kDa. Recombinant human Activin A contains two protein chains of 116 amino acids each and a 6 aa Histidine-based tag for a total length of 122 aa.
• Endotoxin: Endotoxin level is less than 0.05 EU/ug as measured by LAL method.
• Purity: Greater than 95% by SDS page analysis.
• Storage: The lyophilized protein, though stable at 2-8°C in the short term, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Lyophilized protein should be reconstituted in water to a suggested concentration of 25 μg/ml. Due to the protein nature, dimers and multimers may be observed. Not recommended to reconstitute in higher concentration than 25 μg/ml. At higher concentration the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.