Active Recombinant Human ABO, His-tagged

• Cat.No.: ABO-269H
• Product Overview: Recombinant Human ABO (Accession # AAD26574) Arg63-Pro354, fused with an N-terminal human CD33 signal sequence and 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 63-354 a.a.
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer galactose from UDP-galactose to lacto-N-fucopentaose.The specific activity is >1,250 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ABO, His-tagged has a calculated MW of 34.8 kDa. In SDS-PAGE migrates as 35-40 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ABO ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase) [ Homo sapiens ]
• Official Symbol: ABO
• Synonyms: ABO; ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase); histo-blood group ABO system transferase; A3GALNT; A3GALT1; ABO glycosyltransferase; histo-blood group A transferase; histo-blood group B transferase; histo-blood group A2 transferase; B(A) alpha-1,3-galactosyltransferase; fucosylglycoprotein 3-alpha-galactosyltransferase; fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; glycoprotein-fucosylgalactoside alpha-galactosyltransferase; glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; GTB; NAGAT
• Gene ID: 28
• mRNA Refseq: NM_020469
• Protein Refseq: NP_065202
• UniProt ID: P16442
• Chromosome Location: 9q34.2