Active Recombinant Human USP10, Flag-tagged

• Cat.No.: USP10-391H
• Product Overview: Recombinant Human USP10 fused with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.

Specification

• Cat. No.: USP10-391H
• Description: Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene.
• Source: Baculovirus
• Molecular Mass: 88 kDa
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml Flag peptide, 10% glycerol, and 3 mM DTT.
• Purity: ≥60%
• Specific Activity: 23 pmol/min/μg. Assay conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP10 in a 50 μL reaction. Reaction was incubated at 37 °C for 15 min and fluorescent signal was measured at excitation 340 nm, emission 460 nm.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 °C
• OfficialSymbol: USP10
• Protein length: 2-798 a.a.

Gene Information

• Gene Name: USP10 ubiquitin specific peptidase 10 [Homo sapiens]
• Synonyms: ubiquitin specific peptidase 10; Ubiquitin-specific-processing protease 10; KIAA0190; MGC2621; UBPO; ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; Deubiquitinating enzyme 10; EC 3.4.19.12; Ubiquitin thiolesterase 10
• Gene ID: 9100
• mRNA Refseq: NM_005153
• Protein Refseq: NP_005144
• MIM: 609818
• UniProt ID: Q14694
• Chromosome Location: 16q24.1
• Pathway: cysteine-type peptidase activity; cystic fibrosis transmembrane conductance regulator binding; p53 binding; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity