Active Recombinant Full Length Human tryptase alpha/beta 1 Protein, His tagged

• Cat.No.: TPSAB1-13HFL
• Product Overview: Active Recombinant Full Length Human tryptase alpha/beta 1 Protein (Met1-Pro275) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: His
• Protein Length: 1-275 aa
• Description: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.
• Form: Pro form
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 650 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 29 kDa
• N-terminal Sequence Analysis: Val29
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with dry ice or equivalent.
• Reference: 1. Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.

Gene Information

• Gene Name: TPSAB1 tryptase alpha/beta 1 [ Homo sapiens (human) ]
• Official Symbol: TPSAB1
• Synonyms: TPSAB1; tryptase alpha/beta 1; TPS1, TPS2, TPSB1, tryptase beta 1; tryptase alpha/beta-1; tryptase alpha II; tryptase beta I; tryptase I; tryptase II; tryptase III; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta-1; tryptase alpha-1; mast cell beta I tryptase; mast cell alpha II tryptase; TPS1; TPS2; TPSB1; MGC104064
• Gene ID: 7177
• mRNA Refseq: NM_003294
• Protein Refseq: NP_003285
• MIM: 191080
• UniProt ID: Q15661