Active Recombinant Full Length Human SYK Protein, DDK-tagged, Biotinylated

• Cat.No.: SYK-6584HF
• Product Overview: Full-length human SYK [1-635(end) amino acids of accession number NP_003168.2] was expressed as N- terminal DYKDDDDK tagged, biotinylated protein (76 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose and activated with ATP. Activated protein was purified by using gel filtration.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Flag
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-635(end)
• Purity: 98%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: spleen tyrosine kinase
• Official Symbol: SYK
• Synonyms: SYK;p72-Syk
• Protein Refseq: NP_003168.2
• UniProt ID: P43405