Active Recombinant Full Length Human STK39 Protein, GST-tagged

• Cat.No.: STK39-7881HF
• Product Overview: Full-length human STLK3 [1-545(end) amino acids of accession number NP_037365.2] was co-expressed as N-terminal GST-fusion protein (87 kDa) with His-tagged WNK1 [1-491 amino acids of accession number NP_061852.1] using baculovirus expression system. GST-STLK3 was purified by using glutathione sepharose chromatography and Ni-NTA chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein/peptide, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG).
• Protein length: 1-545
• Purity: 93%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: serine threonine kinase 39
• Official Symbol: STK39
• Synonyms: STK39;DCHT;PASK;SPAK
• Protein Refseq: NP_037365.2
• UniProt ID: Q9UEW8