Active Recombinant Full Length Human SOX4 Protein, C-Flag-tagged

• Cat.No.: SOX4-493HFL
• Product Overview: Recombinant Full Length Human SOX4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
• Molecular Mass: 47.1 kDa
• AA Sequence: MVQQTNNAENTEALLAGESSDSGAG LELGIASSPTPGSTASTGGKADDPS WCKTPSGHIKRPMNAFMVWS QIERRKIMEQSPDMHNAEISKRLGK RWKLLKDSDKIPFIREAERLRLKHM ADYPDYKYRPRKKVKSGNAN SSSSAAASSKPGEKGDKVGGSGGGG HGGGGGGGSSNAGGGGGGASGGGAN SKPAQKKSCGSKVAGGAGGG VSKPHAKLILAGGGGGGKAAAAAAA SFAAEQAGAAALLPLGAAADHHSLY KARTPSASASASSAASASAA LAAPGKHLAEKKVKRVYLFGGLGTS SSPVGGVGAGADPSDPLGLYEEEGA GCSPDAPSLSGRSSAASSPA AGRSPADHRGYASLRAASPAPSSAP SHASSSASSHSSSSSSSGSSSSDDE FEDDLLDLNPSSNFESMSLG
  SFSSSSALDRDLDFNFEPGSGSHFE FPDYCTPEVSEMISGDWLESSISNL VFTYSGPTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors

Gene Information

• Gene Name: SOX4 SRY-box transcription factor 4 [ Homo sapiens (human) ]
• Official Symbol: SOX4
• Synonyms: CSS10; EVI16
• Gene ID: 6659
• mRNA Refseq: NM_003107.3
• Protein Refseq: NP_003098.1
• MIM: 184430
• UniProt ID: Q06945

Reviews

08/09/2021

The anti-interference ability in Western blot is very strong, and it can effectively resist the influence of endogenous interference factors.

09/19/2020

The ability to maintain stable performance after multiple uses gives me more confidence in the results.

03/05/2020

SOX4 has a high safety profile and has no adverse reactions during use.

Q&As

How does SOX4 protein interact with other proteins?

SOX4 proteins may interact with other proteins to fulfill their functions. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of SOX4 proteins in cells.

What are the effects of mutations or modifications in the SOX4 protein on organisms?

Mutations or modifications of the SOX4 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

What is the expression pattern of SOX4 protein?

This protein may be highly expressed in specific tissues and cells and low in other tissues and cells. Its expression pattern may be affected by a variety of factors, including gene regulation, transcription factors, etc.

In which biological processes does SOX4 protein function?

SOX4 protein may be involved in a variety of biological processes, including sex determination, gonadal development, nervous system development, etc. These processes require the regulatory action of the SOX4 protein for normal development and function.

How to design experiments to study the structure and function of SOX4 protein?

Studying the structure and function of SOX4 protein may require the design of a series of experiments, including biochemical and cell biology experiments. For example, X-ray crystal diffraction can be used to study its structure, and its function can be studied by gene knockout or knockdown techniques.

How to evaluate the potential of SOX4 protein as a drug target?

Evaluating the potential of SOX4 protein as a drug target can involve several aspects, including its expression level in organisms, its role in biological processes, and its changes in disease. In addition, factors such as ease of drug development for this protein and potential drug side effects need to be considered. At the same time, in vitro and in vivo experiments can be performed with known SOX4 inhibitors or agonists to verify their efficacy and safety.