Active Recombinant Full Length Human RPS6KA4 Protein, GST-tagged

• Cat.No.: RPS6KA4-4448HF
• Product Overview: Full-length human MSK2 [1-772(end) amino acids of accession number NP_003933.1] was co-expressed as N-terminal GST-fusion protein (114 kDa) with human His-tagged Erk2 [1-360 amino acids of accession number NP_002736.3] using baculovirus expression system. GST-MSK2 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-772
• Purity: 76%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: ribosomal protein S6 kinase, 90kDa, polypeptide 4
• Official Symbol: RPS6KA4
• Synonyms: RPS6KA4;MSK2;RSK-B;S6K-alpha-4
• Protein Refseq: NP_003933.1
• UniProt ID: O75676