Active Recombinant Full Length Human PRKAA1 / AMPK (A1/B1/G1) Protein, GST-tagged

• Cat.No.: PRKAA1-2412HF
• Product Overview: Full-length human AMPKα1 [1-550(end) amino acids of accession number NP_006242.4] was co- expressed as N-terminal GST-fusion protein (90 kDa) with GST-PRKAB1 [1-270(end) amino acids of accession number NP_006244.2] and PRKAG1 [1-331(end) amino acids of accession number NP_002724.1] using baculovirus expression system. GST-AMPKα1/β1/γ1 was purified by using glutathione sepharose chromatography and activated with His-tagged CaMKK1. Activated GST- AMPKα1/β1/γ1 was purified using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-550(end)
• Purity: 81%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Official Symbol: PRKAA1
• Synonyms: PRKAA1;AMPK;AMPKa1
• Protein Refseq: NP_006242.4
• UniProt ID: Q13131