Active Recombinant Full Length human PPARG protein, His tagged
Cat.No. : | PPARG-33HFL |
Product Overview : | Active Recombinant Full Length human peroxisome proliferator activated receptor gamma protein with His tag was expressed in E. coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-477 aa |
Description : | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
Tag : | His |
Molecular Mass : | 59.7 kDa |
AA Sequence : | MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY |
Purity : | 0.9 |
Quality Control Testing : | The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified PPAR-gamma is greater than 95% homogeneous based on SDS-PAGE analysis. |
Unit Definition : | 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay. |
Applications : | PPARγ has been applied in DNA and protein-protein interaction assays. |
Storage : | Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles. |
Storage Buffer : | 20mM Tris-HCl pH7.9, 100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. |
References : | 1. Desvergene et al., (1999) Endocr. Rev. 20, 649-688 2. Kersten (2000) Nature 405, 421-424 3. Rosen et al., (1999) Mol. Cell 4, 611-617 |
Gene Name | PPARG peroxisome proliferator activated receptor gamma [ Homo sapiens (human) ] |
Official Symbol | PPARG |
Synonyms | PPARG; peroxisome proliferator activated receptor gamma; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing |
Gene ID | 5468 |
mRNA Refseq | NM_138712 |
Protein Refseq | NP_619726 |
MIM | 601487 |
UniProt ID | D2KUA6 |
◆ Recombinant Proteins | ||
PPARG-804C | Recombinant Cynomolgus PPARG Protein, His-tagged | +Inquiry |
PPARG-5498H | Recombinant Human PPARG protein, His-tagged | +Inquiry |
Pparg-1961M | Recombinant Mouse Pparg Protein, His-tagged | +Inquiry |
PPARG-13162M | Recombinant Mouse PPARG Protein | +Inquiry |
PPARG-28670TH | Recombinant Human PPARG, GST-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
PPARG-492HCL | Recombinant Human PPARG cell lysate | +Inquiry |
Not For Human Consumption!
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