Active Recombinant Full Length human PPARG protein, His tagged

• Cat.No.: PPARG-33HFL
• Product Overview: Active Recombinant Full Length human peroxisome proliferator activated receptor gamma protein with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-477 aa
• Description: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
• Tag: His
• Molecular Mass: 59.7 kDa
• AA Sequence: MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY
• Purity: 0.9
• Quality Control Testing: The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified PPAR-gamma is greater than 95% homogeneous based on SDS-PAGE analysis.
• Unit Definition: 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
• Applications: PPARγ has been applied in DNA and protein-protein interaction assays.
• Storage: Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.
• Storage Buffer: 20mM Tris-HCl pH7.9, 100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• References: 1. Desvergene et al., (1999) Endocr. Rev. 20, 649-688
  2. Kersten (2000) Nature 405, 421-424
  3. Rosen et al., (1999) Mol. Cell 4, 611-617

Gene Information

• Gene Name: PPARG peroxisome proliferator activated receptor gamma [ Homo sapiens (human) ]
• Official Symbol: PPARG
• Synonyms: PPARG; peroxisome proliferator activated receptor gamma; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing
• Gene ID: 5468
• mRNA Refseq: NM_138712
• Protein Refseq: NP_619726
• MIM: 601487
• UniProt ID: D2KUA6