Active Recombinant Full Length Human NEK2 Protein

Cat.No. : NEK2-2744HF
Product Overview : Full-length human NEK2 [1-445(end) amino acids of accession number NP_002488.1] was expressed as N-terminal His-tagged protein (55 kDa) using baculovirus expression system. His-tagged NEK2 was purified by using Ni-NTA affinity chromatography. Purified His-NEK2 was digested by recombinant His- TEV protease, and His-tag free NEK2 (ca. 52 kDa) was collected as flow-through fraction from Ni-NTA affinity chromatography.
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Species : Human
Source : Sf21 Cells
Tag : Non
Protein Length : 1-445
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
Purity : 73%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
Gene Name NIMA (never in mitosis gene a)-related kinase 2
Official Symbol NEK2
Synonyms NEK2;HsPK21;NEK2A;NLK1;PPP1R111;RP67
Protein Refseq NP_002488.1
UniProt ID P51955

Not For Human Consumption!

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