Active Recombinant Full Length Human NEK2 Protein
Cat.No. : | NEK2-2744HF |
Product Overview : | Full-length human NEK2 [1-445(end) amino acids of accession number NP_002488.1] was expressed as N-terminal His-tagged protein (55 kDa) using baculovirus expression system. His-tagged NEK2 was purified by using Ni-NTA affinity chromatography. Purified His-NEK2 was digested by recombinant His- TEV protease, and His-tag free NEK2 (ca. 52 kDa) was collected as flow-through fraction from Ni-NTA affinity chromatography. |
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Species : | Human |
Source : | Sf21 Cells |
Tag : | Non |
Protein Length : | 1-445 |
Form : | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5 |
Bio-activity : | The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. |
Purity : | 73% |
Applications : | SDS-PAGE |
Storage : | Store at -80C. Avoid repeating freeze-thaws. |
Gene Name | NIMA (never in mitosis gene a)-related kinase 2 |
Official Symbol | NEK2 |
Synonyms | NEK2;HsPK21;NEK2A;NLK1;PPP1R111;RP67 |
Protein Refseq | NP_002488.1 |
UniProt ID | P51955 |
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NEK2-2744HF | Active Recombinant Full Length Human NEK2 Protein | +Inquiry |
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NEK2-1183HCL | Recombinant Human NEK2 cell lysate | +Inquiry |
Not For Human Consumption!
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