Active Recombinant Full Length Human Histone Deacetylase 8, His-tagged

• Cat.No.: HDAC8-27529TH
• Product Overview: Recombinant full length protein (Human), C-terminal His tag.

Specification

• Species: Human
• Tag: His
• Protein Length: 1-377aa
• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
• Conjugation: HIS
• Tissue specificity: Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
• Biological activity: 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl,pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30oC.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the histone deacetylase family. HD type 1 subfamily.
• Full Length: Full L.

Gene Information

• Gene Name: HDAC8 histone deacetylase 8 [ Homo sapiens ]
• Official Symbol: HDAC8
• Synonyms: HDAC8; histone deacetylase 8; HDACL1, histone deacetylase like 1; RPD3
• Gene ID: 55869
• mRNA Refseq: NM_001166418
• Protein Refseq: NP_001159890
• MIM: 300269
• Uniprot ID: Q9BY41
• Chromosome Location: Xq13
• Pathway: Cell cycle, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem
• Function: NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; histone deacetylase activity (H3-K16 spe