Active Recombinant Full Length Human Histone Deacetylase 8, His-tagged

• Cat.No.: HDAC8-759H
• Product Overview: Recombinant Human histone deacetylase 8 (HDAC8) was fused to at the C-terminus to a His•Tag sequence at and expressed inS. frugiperdainsect cells using a baculovirus expression system. MW = 46400 Da.

Specification

• Species: Human
• Source: S.frugiperda
• Tag: His
• Protein Length: 1-377aa
• Description: HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
• Purity: ≥90% by SDS-PAGE.
• Form: Liquid. In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN-20 detergent, pH 8.0. Please refer to vial label for lot-specific concentration.
• Specific Activity: ≥10 units/µg protein.
• Unit Definition: One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
• Recommended Reaction Conditions: 137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 100 µM fluorescent substrate [Arg-His-Lys(Ac)-Lys(Ac)], 100 µg/ml BSA, 15 ng/ml HDAC8; incubate at 30°C for 60 min.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70℃. Avoid freeze/thaw. Following reconstitution, aliquot and freeze (-70°C).

Gene Information

• Gene Name: HDAC8 histone deacetylase 8 [ Homo sapiens ]
• Synonyms: HDAC8; histone deacetylase-like 1; RPD3; HDACL1; histone deacetylase 8; EC 3.5.1.98; HD8
• Gene ID: 55869
• mRNA Refseq: NM_018486
• Protein Refseq: NP_060956
• MIM: 300269
• UniProt ID: Q9BY41
• Chromosome Location: Xq13
• Pathway: Gene Expression
• Function: histone deacetylase activity; hydrolase activity; transcription factor binding