Active Recombinant Full Length Human DNMT1 Protein, N-6xHis-FLAG-tagged

• Cat.No.: DNMT1-02H
• Product Overview: Active Recombinant Full Length Human DNMT1 Protein (NP_001370.1) was expressed in Sf9 cells and contains an N-terminal 6xHis Tag and FLAG-Tag.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-6xHis-FLAG
• Form: Supplied in 25 mM HEPES-NaOH pH 7.5, 500 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.
• Bio-activity: Recombinant DNMT1 protein activity assay Assay was performed using DNMT Activity / Inhibition Assay, Active Motif. The substrate, AdoMet and DNMT1 were incubated for 1 hour at 37°C. The whole reactions were developed for 4 min.
• Molecular Mass: 188.2 kDa
• Purity: >90% pure by SDS-PAGE.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DNMT1 DNA (cytosine-5-)-methyltransferase 1 [ Homo sapiens ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA (cytosine-5-)-methyltransferase 1; DNMT; DNA (cytosine-5)-methyltransferase 1; CXXC9; MCMT; m.HsaI; DNA MTase HsaI; CXXC finger protein 9; DNA methyltransferase 1; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9; AIM; HSN1E; FLJ16293; MGC104992
• Gene ID: 1786
• mRNA Refseq: NM_001130823
• Protein Refseq: NP_001124295
• MIM: 126375
• UniProt ID: P26358