Active Recombinant Full Length Human CDK6 Protein, GST-tagged
Cat.No. : | CDK6-6342HF |
Product Overview : | Full-length human CDK6 [1-326(end) amino acids of accession number NP_001250.1] was co-expressed as N-terminal GST-fusion protein (64 kDa) with human GST-CyclinD3 [1-292(end) amino acids of accession number AAA51927.1] using baculovirus expression system. GST-CDK6/CycD3 was purified by using glutathione sepharose chromatography and activated with His-tagged CDK7. Activated GST- CDK6/CycD3 was purified using glutathione sepharose chromatography. |
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Species : | Human |
Source : | Sf21 Cells |
Tag : | GST |
Protein Length : | 1-326(end) |
Form : | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5 |
Bio-activity : | The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000. |
Purity : | 94% |
Applications : | SDS-PAGE |
Storage : | Store at -80C. Avoid repeating freeze-thaws. |
Gene Name | cyclin-dependent kinase 6 |
Official Symbol | CDK6 |
Synonyms | CDK6;MCPH12;PLSTIRE |
Protein Refseq | NP_001250.1 |
UniProt ID | Q00534 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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