Recombinant Human Cyclin-Dependent Kinase 6, His-tagged

• Cat.No.: CDK6-1002H
• Product Overview: Recombinant full-length human CDK6 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 39 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25 % glycerol.
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: B Cell Receptor Signaling Pathway; C-MYB transcription factor network; Cell Cycle; Chronic myeloid leukemia; Coregulation of Androgen receptor activity; Cyclin D associated events in G1; G1 Phase; G1 to S cell cycle control; Glioma; IL2 signaling events mediated by STAT5; Measles; Melanoma; Mitotic G1-G1/S phases; Non-small cell lung cancer; Pancreatic cancer

Gene Information

• Gene Name: CDK6 cyclin-dependent kinase 6 [ Homo sapiens ]
• Official Symbol: CDK6
• Synonyms: CDK6; cyclin-dependent kinase 6; PLSTIRE; MGC59692; OTTHUMP00000162478; OTTHUMP00000208823; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; Cyclin-dependent kinase 6; EC 2.7.11.22
• Gene ID: 1021
• mRNA Refseq: NM_001145306
• Protein Refseq: NP_001138778
• MIM: 603368
• UniProt ID: Q00534
• Chromosome Location: 7q21-q22
• Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding