Active Recombinant Full Length Human AURKB Protein, DDK-tagged, Biotinylated

• Cat.No.: AURKB-4230HF
• Product Overview: Full-length human AurB [1-344(end) amino acids of accession number Q96GD4] was co-expressed as N- terminal DYKDDDDK tagged, biotinylated protein (43 kDa) with His-tagged INCENP(INBOX) [803- 918(end) amino acids of accession number AAU04398] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose and activated with ATP. Activated protein was purified by using gel filtration.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• Protein Length: 1-344(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Purity: 82%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: aurora kinase B
• Official Symbol: AURKB
• Synonyms: AURKB;AIK2;AIM-1;AIM1;ARK-2;ARK2;AurB;IPL1;PPP1R48;STK-1;STK12;STK5;aurkb-sv1;aurkb-sv2;Aik2
• Protein Refseq: Q96GD4
• UniProt ID: Q96GD4