Active Recombinant Full Length Human / AMPK (A1/B2/G1)PRKAA1 Protein, DDK/GST-tagged, Biotinylated

• Cat.No.: PRKAA1-6297HF
• Product Overview: Full-length human AMPKα1 [1-550(end) amino acids of accession number NP_006242.4] was co- expressed as N-terminal DYKDDDDK tagged, biotinylated protein (67 kDa) with GST-PRKAB2 [1- 272(end) amino acids of accession number NP_005390.1] and PRKAG1 [1-331(end) amino acids of accession number NP_002724.1] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose and activated with His-tagged CaMKK1. Activated protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Flag
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-550(end)
• Purity: 100%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Official Symbol: PRKAA1
• Synonyms: PRKAA1;AMPK;AMPKa1
• Protein Refseq: NP_006242.4
• UniProt ID: Q13131